Drag the diffraction-focus knob below the diagram and watch the plane the intermediate lens is imaging slide on and off the back focal plane.
In imaging mode the FOCUS knob drives the objective lens. The moment you press DIFF / D, the same physical knob is remapped to the intermediate (diffraction) lens — on JEOL columns it is literally labelled DIFF FOCUS.
Turning it changes the intermediate-lens current, which slides its object plane up and down the column. Your job is to park that plane exactly on the objective’s back focal plane, because that is where the diffraction pattern physically lives.
Misfocused, the lens hands the projectors a plane near the BFP instead: each Bragg spot becomes a small disc, and at large defocus each disc is actually a tiny shadow image of your selected area.
Sharp focus is not cosmetic: spot radii R₁, R₂ are what you measure for the ratio method (R₂/R₁ = d₁/d₂), so a defocused pattern degrades your indexing — see the SAED indexing guide in this project.